Some researchers have expressed concerns regarding the increased risk of groundwater pollution associated with edta assisted phytoextraction wu et al. This method is to increases mobility of metals in soil by application of chelating agents or surfactants, such as citric acid, edta, cdta, dtpa, egta, eddha, and nta, so that the metals can be taken up more easily by. The heavy metals that plants extract are toxic to the plants as well, and the plants used for phytoextraction are known hyperaccumulators. However, dtpa, edga, and edta have negative impacts that include low biodegradability in soil and increasing the risk of leaching of metals. Edta enhanced phytoextraction of pb from contaminated soil. Chelators including dtpa diethylene triamine pentaacetic acid and oxalic acid were selected for inducing phytoextraction of heavy metals hms from pb, tl, and pbtl contaminated soil, in which heavy metals leakage was highly remarkable. Lead phytoextraction, using plants to extract pb from contaminated soils, is an emerging technology. A number of synthetic chelating agents like dtpa, edta, natural chelating agents like edds, nta. Heavy metal phytoextraction by sedum alfredii is affected.
The role of edta on cadmium phytoextraction in a cadmium hyperaccumul ator rorippa globos a y uebing sun 1,2, qixing zhou 2,3, yingming xu 1, lin w ang 1 and xuefeng liang 1. The effects of edta, dtpa and nta application on solubility of coppercu. Lesage e, meers e, vervaeke p, lamsal s, hopgood m, tack f, verlov m 2005 enhanced phytoextraction. Effect of edta and dtpa on phytoremediation of pbzn. Effect of edta on arsenic phytoextraction by arundo donax l. Increased leaching of pb during edtaassisted phytoextraction has mainly been attributed to the solubility of the pbedta complexes. Comparison of natural organic acids and synthetic chelates. These results showed that the application of edta can be a useful strategy for phytoextraction of cu by b. Chemically enhanced phytoextraction of pb by wheat in texturally different soils saifullaha,b, munir hussain ziac,d, erik meerse, abdul ghafoora, ghulam murtazaa, muhammad sabira, muhammad ziaurrehmana, f.
Chelating agents work by binding and holding on to radioactive materials or poisons that get into the body. The plants used for phytoextraction of toxic metals from soil are expected to have two important. Hence, phytoextraction can be a perfect technique for soil purification because of its minimal limitation. Some researchers have expressed concerns regarding the increased risk of groundwater pollution associated with edtaassisted phytoextraction wu et al. Aug 28, 2014 copper cu is an essential micronutrient for normal plant growth and development, but in excess, it is also toxic to plants.
To our knowledge, such natural aromatic acids as vanillic and gallic acids have not been tested previously for chemically assisted phytoextraction. Dec 24, 2019 however, dtpa, edga, and edta have negative impacts that include low biodegradability in soil and increasing the risk of leaching of metals into groundwater 21, 22. Induced phytoextraction of lead from contaminated soils by. Chelating agents either synthetic edta, dtpa, hedta or natural oxalic acid, citric acid, acetic acid are commonly used as amendments to induced phytoextraction 22. Effect of chelating agents on heavy metal extraction from contaminated soils shazia akhtar, shazia iram. Enhanced phytoextraction of edta and edds in polluted soil. Preparation of standards and sample solutions for dtpa determination a dtpa standard solution was prepared by dissolving 39. Edta december 2, 2010 by oliver in chemistry, conservation treatments, outdoor sculpture. However, dtpa, edga, and edta have negative impacts that include low biodegradability in soil and increasing the risk of leaching of metals into groundwater 21, 22. The tailing ponds of leadzinc mines are artificial environment pollution sources, and also important dangerous sources of heavy metal contamination in leadzinc mining areas. Effect of edta and citric acid on heavy metal uptake by helianthus annuus from a calcareous soil. In this communication, basic concepts relating to phytoextraction of metalpolluted soils are discussed as well as some limitations to and perspectives. Chelantinduced phytoextraction of heavy metals from. Whereas reverse trend was observed in nta, ca and dtpa treated soils.
Contaminated soils by eucalyptus camaldulensis dehnh and. Enhanced phytoremediation of heavy metal polluted soil with native crops of punjab, pakistan shazia akhtar. Edta, a synthetic chelator, biodegrades poorly in soil because of its effectiveness at complexing metals. Effect of chelating agents on heavy metal extraction from.
Effect of biodegradable chelators on induced phytoextraction. Chelateassisted phytoextraction of cadmium from a mine. Roles of edta and dtpa treatment time mahdieh ebrahimi1, mohammad jafari2, alitavili3 1 department of range and watershed management, university of zabol, zabol, iran. Pbphytoextraction by maize in a pbedta treated oxisol. Pb availability, uptake, and translocation constraints j. Both edta and dtpa resulted in increased levels of heavy metals in the aboveground biomass. Pdf edta assisted phytoremediation of cadmium, lead and zinc. At the end of the experiment, shoot accumulation of cadmium, chromium, copper, iron, manganese, nickel, lead, and. Pdf the role of edta on cadmium phytoextraction in a. Chelating agents of a new generation as an alternative. The objectives of this study are to investigate the phytoextraction. Oct 11, 2006 diethylenetriamene pentaacetate dtpa what is dtpa. In order to minimum high environmental persistence while enhancing the effects of phytoextract heavy metals in plant, though applied different ratio of nonbiological chelating agent edta and biological chelating agent edds application phytoremediation into the rape brassica campestris l, monocots corn zea mays l. Leaching and uptake of heavy metals by ten different.
Dtpa is a kind of medicine called a chelating agent. In this communication, basic concepts relating to phytoextraction of metalpolluted soils are discussed as well as some limitations to and perspectives for applying this method in the region are considered. Mung bean and buckwheat had a higher sensitivity to the edta treatment in soils. Chemically enhanced phytoextraction of pb by wheat in.
Chelating agents, nickel, phytoextraction, zea mays. This experiment was conducted with a simulation in pb, cu, cd complex. Chelate assisted phytoextraction using oilseed brassicas. Although, edta amendment with cu significantly increased cu uptake in roots, stems, and leaves in decreasing order of concentration but increased the growth, photosynthetic parameters, and antioxidant enzymes. One of the recurring needs of the cantor arts centers outdoor art collection is the removal of mineral deposits from metal sculptures. Phytoextraction is a subprocess of phytoremediation in which plants remove dangerous elements or compounds from soil or water, most usually heavy metals, metals that have a high density and may be toxic to organisms even at relatively low concentrations. A scientific journal of comsats science vision vol. Phytoextraction is a technique that uses plants able to accumulate high quantities of hm in their tissues. Phytoextraction ability of the grass pennisetum pedicellatum under the influence of different level of applied chelator. Phytoextraction is a costeffective method that could be an alternative to remediate polluted sites in latin america. A number of chelating agents, for example eddha, edds, cdta, dtpa, hedta, egta, edta and. Insights into heavy metals leakage in chelatorinduced. Ministry of education key laboratory of environmental remediation and ecosystem health, zhejiang university, hangzhou 310029.
For shacking time significant results were achieved at 120 hours by applying edta and nta where as dtpa. Pb is one of the most widespread and metal pollutants in soil. Ionpairing hplc methods to determine edta and dtpa in small. The present study investigated the influence of ethylenediaminetetraacetic acid edta in enhancing cu uptake and tolerance as well as the morphological and physiological responses of brassica napus l. Edtaenhanced phytoextraction of pb from contaminated soil. The soil had been contaminated with cu for more than 3 years before the pot experiment was conducted, hence the nh 4 no 3extractable fraction was only 0. This chapter will focus on the phytoextraction mechanism, metal uptake, and accumulation.
Considering these negative effects, edds and ca have been widely used to increase the capacity of metals translocation from contaminated soil to harvestable parts of the tested. As for pb, the content of pb in root and shoot reached a maximum of 2730. Once bound to a radioactive material or poison, the chelating agent is then passed from the body in the urine. Edta and dtpa significantly enhanced the contents of zn and pb in ryegrass as compared with the control. Both edta and dtpa resulted in increased levels of heavy metals in the above ground biomass. It was found that with increasing chelating agent doses metals availability was increased and 5. Applied doses varied between treatments based on plant tolerance.
It is generally concentrated in surface layers with only. Chelateassisted phytoextraction of cadmium from a mine soil. Tacke a institute of soil and environmental sciences, university of agriculture, faisalabad38040, pakistan bsoil and water science department, institute of food science and. Edta, dtpa, edds and ca, two metals cu and zn, and four plants sunflower, chinese cabbage, cattail, and reed in high and low organic content soil in order to compare phytoextraction efficiency. Phytoextraction was primarily employed to recover heavy metals from soils, however, this technology is now applicable to other materials in different media. Edta enhanced plant growth, antioxidant defense system. Phytoextraction of lead from contaminated soils by panicum virgatum, enhanced with edta, citric acid, benomyl, propiconazole and nitric oxide by adrianna beavers a thesis presented in partial fulfillment of requirements for the master of science in integrative biology for the department of ecology, evolution, and organismal biology. Calculations of soil pb mass balance suggest that this technology will be economically feasible only if systems can be developed to employ high biomass plants that can accumulate greater than 1% pb in their shoots. Improved phytoextraction capacity of prosopiscineraria l. The chelating agent under environmental scrutiny article pdf available in quimica nova 266.
Review chelantinduced phytoextraction of heavy metals from. Enhanced phytoremediation of heavy metal polluted soil. Ameliorative effect of chelating agents on photosynthetic. Effect of chelating agents on phytoextraction of ni from. Nh 4 no 3 is a weak extractant that extracts mainly the watersoluble and part of the exchangeable fraction of metals. Comparison of natural organic acids and synthetic chelates at. Chelatorenhanced phytoextraction of copper and zinc by. Increased leaching of pb during edta assisted phytoextraction has mainly been attributed to the solubility of the pb edta complexes. Effects of edta and dtpa on lead and zinc accumulation of. Effect of biodegradable chelators on induced phytoextraction of. In this study, we investigated the potential of adding chelates to pb. Edds was less phytotoxic than edta but the results were statistically. Effect of edta and dtpa on phytoremediation of pbzn contaminated soils by eucalyptus camaldulensis dehnh and effect on treatment time. For the treatments with edta, the saturation extract soil solution was the less effective in predicting the soilpb availability as compared to dtpa and mehlich3 extracts.
Ionpairing hplc methods to determine edta and dtpa in. The leaching risk of tl and pb during the chelatorinduced phytoextraction was highly concerned, providing important information on understanding the application of chelatorinduced phytoextraction in pb. Edta enhanced plant growth, antioxidant defense system, and. In the present study shacking and incubation experiment were carried out in order to evaluate the changes in heavy metal solubility in the studied soils by addition of different concentration of ethylene dinitrilo tetra acetic acid edta, diethylene triamine penta acetic acid dtpa, and nitrilo tri acetic acid nta. Edta and citric acid mediated phytoextraction of zn, cu, pb. The state of scientific knowledge and research needs on.
Heavy metal phytoextraction by sedum alfredii is a. These findings are consistent with a previous study that did not show correlation between the soil solutionpb and the one found in wheat leaves lee et al. Nta, have been investigated for their capability to dissolve. The leaching risk of tl and pb during the chelatorinduced phytoextraction was highly concerned, providing important information on understanding the application of chelatorinduced phytoextraction in pb and tlcontaminated soil remediation. Phytoremediation of heavy metal contaminated soil is a developing technology that aims to extract or inactivate metals and it has attracted much attention because it is an environmentally friendly and relatively cheap technique mcgrath, 1998, mcgrath et al.
Study on application of phytoremediation technology in. Cu, pb, cd and cr were solubilized by dtpa extractant. Review chelantinduced phytoextraction of heavy metals. Edtaenhanced phytoremediation of heavy metal contaminated.
One is aminopolycarboxylic acids apcas, such as ethylene diamine tetraacetic acid edta, ethylene bisoxyethylenetrinitrilo tetraacetic acid egta, diethylene triamino pentaacetic acid dtpa. Greenhousebased hydroponic systems using plants with high contaminant root uptake and poor translocation to the shoots are currently being researched for removal of heavy metals and. However, during edtaassisted pb phytoextraction, there are several factors that influence plant growth, among which the most important are. To study the effects of ethylene diamine tetracetic acid edta and diethylene triamine penlaacetic acid dtpa on phytoremediation of leadzinc mining area soil, two chelators edta and dtpa were used in. Pdf enhanced phytoextraction proposes the use of soil amendments to increase the heavymetal content of aboveground harvestable plant tissues. Edta and citric acid mediated phytoextraction of zn, cu. Edta is the most extensively used synthetic chelator for induced phytoextraction which dissolves the bonds between the metal and soil. Results showed that compared with the control group without chelating agent under planting conditions, the extraction efficiency i. Chelants used in the phytoextraction of heavy metals from contaminated soils can be divided into 2 categories. Unlike edta, edds, which is an edta isomer, is a biodegradable chelator. Copper cu is an essential micronutrient for normal plant growth and development, but in excess, it is also toxic to plants. Effect of chelating agents on heavy metal extraction from contaminated soils akhtar shazia 1.
248 1113 1501 740 578 1384 499 1184 628 928 915 332 846 1470 355 143 822 236 1324 33 691 386 662 548 329 1003 972 1227